EPH Receptor B3 Kit ELISA

N° du produit ABIN4882754

Détail du produit EPH Receptor B3 Kit ELISA

Antigène: EPH Receptor B3 (EPHB3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human EphB3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor B3 (EPHB3)

Autre désignation: EphB3 (EPHB3 Produits)

Synonymes: tck Kit ELISA, MGC83457 Kit ELISA, EPHB3 Kit ELISA, ETK2 Kit ELISA, HEK2 Kit ELISA, TYRO6 Kit ELISA, AW456895 Kit ELISA, Cek10 Kit ELISA, Etk2 Kit ELISA, MDK5 Kit ELISA, Sek4 Kit ELISA, Tyro6 Kit ELISA, CEK10 Kit ELISA, EK10 Kit ELISA, ek3 Kit ELISA, ephb3 Kit ELISA, fc62d03 Kit ELISA, rtk3 Kit ELISA, wu:fc62d03 Kit ELISA, zek3 Kit ELISA, EPH receptor B3 Kit ELISA, EPH receptor B3 L homeolog Kit ELISA, Eph receptor B3 Kit ELISA, eph receptor B3a Kit ELISA, Eph receptor tyrosine kinase Kit ELISA, EPHB3 Kit ELISA, ephb3.L Kit ELISA, ephb3 Kit ELISA, Ephb3 Kit ELISA, ephb3a Kit ELISA

ID gène: 2049

UniProt: P54753

Pathways: Signalisation RTK