EPH Receptor B4 Kit ELISA

N° du produit ABIN4882757

Détail du produit EPH Receptor B4 Kit ELISA

Antigène: EPH Receptor B4 (EPHB4)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human EphB4 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human EphB4

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor B4 (EPHB4)

Autre désignation: EphB4 (EPHB4 Produits)

Synonymes: HTK Kit ELISA, MYK1 Kit ELISA, TYRO11 Kit ELISA, htk Kit ELISA, myk1 Kit ELISA, ephB4a Kit ELISA, tyro11 Kit ELISA, ephb4b Kit ELISA, MGC130997 Kit ELISA, AI042935 Kit ELISA, Htk Kit ELISA, MDK2 Kit ELISA, Myk1 Kit ELISA, Tyro11 Kit ELISA, cb646 Kit ELISA, ephB4 Kit ELISA, rtk5 Kit ELISA, EphB-rtk8 Kit ELISA, cb417 Kit ELISA, rtk8 Kit ELISA, EPH receptor B4 Kit ELISA, EPH receptor B4 L homeolog Kit ELISA, EPH receptor B4 S homeolog Kit ELISA, Eph receptor B4 Kit ELISA, eph receptor B4a Kit ELISA, eph receptor B4b Kit ELISA, EPHB4 Kit ELISA, ephb4.L Kit ELISA, ephb4.S Kit ELISA, ephb4 Kit ELISA, Ephb4 Kit ELISA, ephb4a Kit ELISA, ephb4b Kit ELISA

ID gène: 2050

UniProt: P54760

Pathways: Signalisation RTK