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ERBB4 Kit ELISA
ERBB4
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.2-50 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4882769
Détail du produit ERBB4 Kit ELISA
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Antigène
Voir toutes ERBB4 Kits ELISA
ERBB4
(V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
Reactivité
Toutes les réactivités sur ERBB4 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.2-50 ng/mL
Seuil minimal de détection
0.2 ng/mL
Application
ELISA
Fonction
Human ErbB4 (HER4) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Human Receptor Tyrosine Protein Kinase ErbB4 (ERBB4)
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes ERBB4 Kits ELISA
ERBB4
(V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
Autre désignation
ErbB4 (ERBB4 Produits )
Synonymes
erbb4 Kit ELISA, si:ch211-227n20.1 Kit ELISA, Her4 Kit ELISA, c-erbB-4 Kit ELISA, HER4 Kit ELISA, p180erbB4 Kit ELISA, erb-b2 receptor tyrosine kinase 4 Kit ELISA, erb-b2 receptor tyrosine kinase 4a Kit ELISA, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Kit ELISA, erb-b2 receptor tyrosine kinase 4 L homeolog Kit ELISA, receptor tyrosine-protein kinase ErbB-4 Kit ELISA, ERBB4 Kit ELISA, erbb4a Kit ELISA, erbb4 Kit ELISA, erbb4.L Kit ELISA, Tsp_13582 Kit ELISA, Erbb4 Kit ELISA
ID gène
2066
UniProt
Q15303
Pathways
Signalisation RTK , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway
Vus récemment
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