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ERBB4 Kit ELISA

ERBB4 Reactivité: Humain Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4882769
  • Antigène Voir toutes ERBB4 Kits ELISA
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Reactivité
    • 5
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.2-50 ng/mL
    Seuil minimal de détection
    0.2 ng/mL
    Application
    ELISA
    Fonction
    Human ErbB4 (HER4) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human Receptor Tyrosine Protein Kinase ErbB4 (ERBB4)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ERBB4 Kits ELISA
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Autre désignation
    ErbB4 (ERBB4 Produits)
    Synonymes
    erbb4 Kit ELISA, si:ch211-227n20.1 Kit ELISA, Her4 Kit ELISA, c-erbB-4 Kit ELISA, HER4 Kit ELISA, p180erbB4 Kit ELISA, erb-b2 receptor tyrosine kinase 4 Kit ELISA, erb-b2 receptor tyrosine kinase 4a Kit ELISA, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Kit ELISA, erb-b2 receptor tyrosine kinase 4 L homeolog Kit ELISA, receptor tyrosine-protein kinase ErbB-4 Kit ELISA, ERBB4 Kit ELISA, erbb4a Kit ELISA, erbb4 Kit ELISA, erbb4.L Kit ELISA, Tsp_13582 Kit ELISA, Erbb4 Kit ELISA
    ID gène
    2066
    UniProt
    Q15303
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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