EXTL3 Kit ELISA

N° du produit ABIN4882781

Détail du produit EXTL3 Kit ELISA

Antigène: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Exostosin-like 3/EXTL3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TRP14.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Autre désignation: Exostosin-Like 3 (EXTL3 Produits)

Synonymes: BcDNA:LD21192 Kit ELISA, Botv Kit ELISA, CG15110 Kit ELISA, DEXT3 Kit ELISA, DEXTL3 Kit ELISA, Dextl3 Kit ELISA, Dmel\\CG15110 Kit ELISA, Extl3 Kit ELISA, 2900009G18Rik Kit ELISA, AU040105 Kit ELISA, Ext1l Kit ELISA, mKIAA0519 Kit ELISA, BOTV Kit ELISA, EXTL1L Kit ELISA, EXTR1 Kit ELISA, REGR Kit ELISA, RPR Kit ELISA, Regr Kit ELISA, brother of tout-velu Kit ELISA, exostoses (multiple)-like 3 Kit ELISA, exostosin like glycosyltransferase 3 Kit ELISA, exostosin-like glycosyltransferase 3 Kit ELISA, botv Kit ELISA, Extl3 Kit ELISA, EXTL3 Kit ELISA

ID gène: 2137

UniProt: O43909

Pathways: Glycosaminoglycan Metabolic Process, ER-Nucleus Signaling