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FGFBP1 Kit ELISA

FGFBP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882876
  • Antigène Voir toutes FGFBP1 Kits ELISA
    FGFBP1 (Fibroblast Growth Factor Binding Protein 1 (FGFBP1))
    Reactivité
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human FGF-BP (FGFBP1) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human FGF-BP (FGFBP1)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FGFBP1 Kits ELISA
    FGFBP1 (Fibroblast Growth Factor Binding Protein 1 (FGFBP1))
    Autre désignation
    FGF-BP (FGFBP1 Produits)
    Synonymes
    MGC84401 Kit ELISA, fgfbp Kit ELISA, hbp17 Kit ELISA, FGF-BP Kit ELISA, FGF-BP1 Kit ELISA, FGFBP Kit ELISA, FGFBP-1 Kit ELISA, HBP17 Kit ELISA, fibroblast growth factor binding protein 1 S homeolog Kit ELISA, fibroblast growth factor binding protein 1 Kit ELISA, fibroblast growth factor-binding protein 1 Kit ELISA, fgfbp1.S Kit ELISA, FGFBP1 Kit ELISA, fgfbp1 Kit ELISA, Fgfbp1 Kit ELISA, LOC100726816 Kit ELISA, LOC101106941 Kit ELISA
    ID gène
    9982
    UniProt
    Q14512
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