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GAD Kit ELISA
GAD1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882963
Détail du produit GAD Kit ELISA
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Antigène
Voir toutes GAD (GAD1) Kits ELISA
GAD (GAD1)
(Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
Reactivité
Toutes les réactivités sur GAD Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human GAD1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes GAD (GAD1) Kits ELISA
GAD (GAD1)
(Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
Autre désignation
GAD1 (GAD1 Produits )
Synonymes
CPSQ1 Kit ELISA, GAD Kit ELISA, SCP Kit ELISA, GAD-67 Kit ELISA, cpsq1 Kit ELISA, gad1 Kit ELISA, gad1-A Kit ELISA, gad67 Kit ELISA, GAD67 Kit ELISA, GAD1 Kit ELISA, GLUTAMATE DECARBOXYLASE Kit ELISA, GLUTAMATE DECARBOXYLASE 1 Kit ELISA, MKP11.30 Kit ELISA, MKP11_30 Kit ELISA, glutamate decarboxylase Kit ELISA, EP10 Kit ELISA, GAD25 Kit ELISA, GAD44 Kit ELISA, Gad-1 Kit ELISA, Gad67 Kit ELISA, glutamate decarboxylase 1 Kit ELISA, glutamate decarboxylase 1 L homeolog Kit ELISA, glutamate decarboxylase Kit ELISA, glutamate decarboxylase 1b Kit ELISA, GAD1 Kit ELISA, gad1.1.L Kit ELISA, GAD Kit ELISA, Gad1 Kit ELISA, gad1b Kit ELISA
ID gène
2571
UniProt
Q99259
Vus récemment
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