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LGALS1/Galectin 1 Kit ELISA
LGALS1
Reactivité: Humain
Colorimetric
Sandwich ELISA
4.10-1.000 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4882969
Détail du produit LGALS1/Galectin 1 Kit ELISA
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Antigène
Voir toutes LGALS1/Galectin 1 (LGALS1) Kits ELISA
LGALS1/Galectin 1 (LGALS1)
(Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
Reactivité
Toutes les réactivités sur LGALS1/Galectin 1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
4.10-1.000 ng/mL
Seuil minimal de détection
4.10 ng/mL
Application
ELISA
Fonction
Human Galectin-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1, BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1, CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, GITR Ligand, Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes LGALS1/Galectin 1 (LGALS1) Kits ELISA
LGALS1/Galectin 1 (LGALS1)
(Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
Autre désignation
Galectin-1 (LGALS1 Produits )
Synonymes
GAL1 Kit ELISA, GBP Kit ELISA, Galectin-1 Kit ELISA, LGAS1 Kit ELISA, LGALS1 Kit ELISA, MGC64502 Kit ELISA, GB10026 Kit ELISA, AA410090 Kit ELISA, Gal-1 Kit ELISA, Galbp Kit ELISA, L-14.5 Kit ELISA, L14 Kit ELISA, Lect14 Kit ELISA, galectin-1 Kit ELISA, Galaptin Kit ELISA, galectin 1 Kit ELISA, galectin-1 Kit ELISA, lectin, galactoside-binding, soluble, 1 L homeolog Kit ELISA, protein enabled Kit ELISA, lectin, galactose binding, soluble 1 Kit ELISA, LGALS1 Kit ELISA, Lgals1 Kit ELISA, CC1G_05003 Kit ELISA, CC1G_05004 Kit ELISA, lgals1.L Kit ELISA, LOC408848 Kit ELISA
ID gène
3956
UniProt
P09382
Pathways
Carbohydrate Homeostasis
Vus récemment
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