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WFIKKN2 Kit ELISA
WFIKKN2
Reactivité: Humain
Colorimetric
Sandwich ELISA
20-5000 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4883009
Détail du produit WFIKKN2 Kit ELISA
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Antigène
Voir toutes WFIKKN2 Kits ELISA
WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Reactivité
Toutes les réactivités sur WFIKKN2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
20-5000 pg/mL
Seuil minimal de détection
20 pg/mL
Application
ELISA
Fonction
Human GASP-1 (WFIKKNRP) ELISA Kit for serum, plasma, and cell culture supernatant samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples10 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes WFIKKN2 Kits ELISA
WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Autre désignation
GASP-1///WFIKKNRP (WFIKKN2 Produits )
Synonymes
GASP-1 Kit ELISA, WFDC20B Kit ELISA, WFIKKNRP Kit ELISA, hGASP-1 Kit ELISA, 2610304F08Rik Kit ELISA, AY100450 Kit ELISA, Gasp1 Kit ELISA, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 Kit ELISA, WFIKKN2 Kit ELISA, Wfikkn2 Kit ELISA
ID gène
9737
UniProt
Q5JY77
Vus récemment
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