GATA2 Kit ELISA

N° du produit ABIN4883020

Détail du produit GATA2 Kit ELISA

Antigène: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human GATA-2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GATA2 (GATA Binding Protein 2 (GATA2))

Autre désignation: GATA-2 (GATA2 Produits)

Synonymes: DCML Kit ELISA, MONOMAC Kit ELISA, NFE1B Kit ELISA, Gata-2 Kit ELISA, xgata-2 Kit ELISA, xgata2 Kit ELISA, GATA-2 Kit ELISA, NF-E1b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2 Kit ELISA, zg2 Kit ELISA, zgc:91840 Kit ELISA, GATA binding protein 2 Kit ELISA, GATA binding protein 2 L homeolog Kit ELISA, amidase Kit ELISA, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A Kit ELISA, hypothetical protein Kit ELISA, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) Kit ELISA, GATA2 transcription factor Kit ELISA, putative gata binding factor Kit ELISA, GATA binding protein 2a Kit ELISA, GATA binding protein 2b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2.L Kit ELISA, RR_RS20245 Kit ELISA, gatA2 Kit ELISA, Smp_018590 Kit ELISA, gata2a Kit ELISA, gata2b Kit ELISA

ID gène: 2624

UniProt: P23769

Pathways: Stem Cell Maintenance