GCK Kit ELISA

N° du produit ABIN4883035

Détail du produit GCK Kit ELISA

Antigène: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Glucokinase/GCK ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GCK (Glucokinase (Hexokinase 4) (GCK))

Autre désignation: Glucokinase (GCK Produits)

Synonymes: FGQTL3 Kit ELISA, GK Kit ELISA, GLK Kit ELISA, HHF3 Kit ELISA, HK4 Kit ELISA, HKIV Kit ELISA, HXKP Kit ELISA, LGLK Kit ELISA, MODY2 Kit ELISA, Gk Kit ELISA, Gls006 Kit ELISA, GCK Kit ELISA, BA4487 Kit ELISA, glk Kit ELISA, hk4 Kit ELISA, hhf3 Kit ELISA, hkiv Kit ELISA, hxkp Kit ELISA, gck-A Kit ELISA, mody2 Kit ELISA, glucokinase Kit ELISA, GLUKA Kit ELISA, RNGK2 Kit ELISA, glucokinase Kit ELISA, glucokinase (hexokinase 4) L homeolog Kit ELISA, glucokinase (hexokinase 4) Kit ELISA, GCK Kit ELISA, Gck Kit ELISA, glcK Kit ELISA, Bphyt_1025 Kit ELISA, Halhy_6548 Kit ELISA, gk Kit ELISA, gck.L Kit ELISA, gck Kit ELISA

ID gène: 2645

UniProt: P35557

Pathways: Signalisation MAPK, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process