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GFRA2 Kit ELISA
GFRA2
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883060
Détail du produit GFRA2 Kit ELISA
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Antigène
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GFRA2
(GDNF Family Receptor alpha 2 (GFRA2))
Reactivité
Toutes les réactivités sur GFRA2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human GFR alpha-2 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human GFR alpha-2. Other species not yet determined.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes GFRA2 Kits ELISA
GFRA2
(GDNF Family Receptor alpha 2 (GFRA2))
Autre désignation
GFR alpha-2 (GFRA2 Produits )
Synonymes
GDNFRB Kit ELISA, NRTNR-ALPHA Kit ELISA, NTNRA Kit ELISA, RETL2 Kit ELISA, TRNR2 Kit ELISA, Retl2 Kit ELISA, NTNRALPHA Kit ELISA, ntnra Kit ELISA, retl2 Kit ELISA, trnr2 Kit ELISA, gdnfrb Kit ELISA, nrtnr-alpha Kit ELISA, gfra2 Kit ELISA, GDNF family receptor alpha 2 Kit ELISA, glial cell line derived neurotrophic factor family receptor alpha 2 Kit ELISA, GDNF family receptor alpha 2a Kit ELISA, GFRA2 Kit ELISA, Gfra2 Kit ELISA, gfra2 Kit ELISA, gfra2a Kit ELISA
ID gène
2675
UniProt
O00451
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