GLI3 Kit ELISA

N° du produit ABIN4883079

Détail du produit GLI3 Kit ELISA

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human GLI-3.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI-3 (GLI3 Produits)

Synonymes: ACLS Kit ELISA, GCPS Kit ELISA, GLI3-190 Kit ELISA, GLI3FL Kit ELISA, PAP-A Kit ELISA, PAPA Kit ELISA, PAPA1 Kit ELISA, PAPB Kit ELISA, PHS Kit ELISA, PPDIV Kit ELISA, xgli3 Kit ELISA, AI854843 Kit ELISA, AU023367 Kit ELISA, Bph Kit ELISA, Pdn Kit ELISA, Xt Kit ELISA, add Kit ELISA, im:7142603 Kit ELISA, GLI3 Kit ELISA, GLI family zinc finger 3 Kit ELISA, GLI family zinc finger 3 L homeolog Kit ELISA, GLI-Kruppel family member GLI3 Kit ELISA, GLI3 Kit ELISA, Gli3 Kit ELISA, gli3.L Kit ELISA, gli3 Kit ELISA

ID gène: 2737

UniProt: P10071

Pathways: Signalisation Hedgehog