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IL27 Receptor alpha Kit ELISA

IL27RA Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883399
  • Antigène Voir toutes IL27 Receptor alpha (IL27RA) Kits ELISA
    IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human IL-27 Receptor alpha (IL27RA) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human IL-27 Receptor alpha (IL27RA)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes IL27 Receptor alpha (IL27RA) Kits ELISA
    IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
    Autre désignation
    IL-27 R alpha (IL27RA Produits)
    Synonymes
    IL27RA Kit ELISA, CRL1 Kit ELISA, IL-27RA Kit ELISA, IL27R Kit ELISA, TCCR Kit ELISA, WSX1 Kit ELISA, zcytor1 Kit ELISA, IL-27R Kit ELISA, Tccr Kit ELISA, WSX-1 Kit ELISA, Wsx1 Kit ELISA, interleukin 27 receptor subunit alpha Kit ELISA, interleukin-27 receptor subunit alpha Kit ELISA, interleukin 27 receptor, alpha Kit ELISA, IL27RA Kit ELISA, LOC484907 Kit ELISA, Il27ra Kit ELISA
    ID gène
    9466
    UniProt
    Q6UWB1
    Pathways
    Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
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