ITGB1 Kit ELISA

N° du produit ABIN4883481

Détail du produit ITGB1 Kit ELISA

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Integrin beta 1/CD29 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: Integrin beta 1 (ITGB1 Produits)

Synonymes: CD29 Kit ELISA, FNRB Kit ELISA, GPIIA Kit ELISA, MDF2 Kit ELISA, MSK12 Kit ELISA, VLA-BETA Kit ELISA, VLAB Kit ELISA, 4633401G24Rik Kit ELISA, AA409975 Kit ELISA, AA960159 Kit ELISA, ENSMUSG00000051907 Kit ELISA, Fnrb Kit ELISA, Gm9863 Kit ELISA, gpIIa Kit ELISA, cd29 Kit ELISA, fnrb Kit ELISA, gpiia Kit ELISA, itgb1 Kit ELISA, mdf2 Kit ELISA, msk12 Kit ELISA, vla-beta Kit ELISA, vlab Kit ELISA, itgb1-a Kit ELISA, itgb1-b Kit ELISA, integrin subunit beta 1 Kit ELISA, integrin beta 1 (fibronectin receptor beta) Kit ELISA, integrin subunit beta 1 S homeolog Kit ELISA, integrin beta 1 Kit ELISA, integrin subunit beta 1 L homeolog Kit ELISA, ITGB1 Kit ELISA, Itgb1 Kit ELISA, itgb1.S Kit ELISA, LOC100009173 Kit ELISA, itgb1.L Kit ELISA

ID gène: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome