JAM2 Kit ELISA

N° du produit ABIN4883495

Détail du produit JAM2 Kit ELISA

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Junctional Adhesion Molecule B (JAM2 / VE-JAM / CD322) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Junctional Adhesion Molecule B (JAM2 / VE-JAM / CD322)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Autre désignation: JAM-B (JAM2 Produits)

Synonymes: JAM2 Kit ELISA, cd322 Kit ELISA, jam-b Kit ELISA, jamb Kit ELISA, pro245 Kit ELISA, ve-jam Kit ELISA, vejam Kit ELISA, jam2 Kit ELISA, sb:cb1002 Kit ELISA, im:7155552 Kit ELISA, sc:d0813 Kit ELISA, si:ch211-160b11.5 Kit ELISA, LOC100227766 Kit ELISA, 1110002N23Rik Kit ELISA, 2410030G21Rik Kit ELISA, 2410167M24Rik Kit ELISA, JAM-2 Kit ELISA, JAM-B Kit ELISA, Jcam2 Kit ELISA, VE-JAM Kit ELISA, C21orf43 Kit ELISA, CD322 Kit ELISA, JAMB Kit ELISA, PRO245 Kit ELISA, VEJAM Kit ELISA, junctional adhesion molecule 2 Kit ELISA, junctional adhesion molecule 2a Kit ELISA, junctional adhesion molecule 2b Kit ELISA, junction adhesion molecule 2 Kit ELISA, JAM2 Kit ELISA, jam2 Kit ELISA, jam2a Kit ELISA, jam2b Kit ELISA, Jam2 Kit ELISA

ID gène: 58494

UniProt: P57087