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PSMB8 Kit ELISA
PSMB8
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883626
Détail du produit PSMB8 Kit ELISA
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Antigène
Voir toutes PSMB8 Kits ELISA
PSMB8
(Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))
Reactivité
Toutes les réactivités sur PSMB8 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human LMP7/PSMB8 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes PSMB8 Kits ELISA
PSMB8
(Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))
Autre désignation
LMP7 (PSMB8 Produits )
Synonymes
ALDD Kit ELISA, D6S216 Kit ELISA, D6S216E Kit ELISA, JMP Kit ELISA, LMP7 Kit ELISA, NKJO Kit ELISA, PSMB5i Kit ELISA, RING10 Kit ELISA, ATP6V0D2 Kit ELISA, lmp7 Kit ELISA, prosome Kit ELISA, macropain Kit ELISA, Lmp-7 Kit ELISA, Lmp7 Kit ELISA, Ring10 Kit ELISA, proteasome subunit beta 8 Kit ELISA, proteasome subunit beta 8A Kit ELISA, proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) Kit ELISA, PSMB8 Kit ELISA, psmb8a Kit ELISA, Psmb8 Kit ELISA
ID gène
5696
Pathways
Mitotic G1-G1/S Phases , DNA Replication , Synthesis of DNA
Vus récemment
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