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LRRN1 Kit ELISA

LRRN1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883658
  • Antigène Voir toutes LRRN1 Kits ELISA
    LRRN1 (Leucine Rich Repeat Neuronal 1 (LRRN1))
    Reactivité
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human LRRN1/NLRR-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes LRRN1 Kits ELISA
    LRRN1 (Leucine Rich Repeat Neuronal 1 (LRRN1))
    Autre désignation
    LRRN1 (LRRN1 Produits)
    Synonymes
    lrrn1 Kit ELISA, nlrr-1 Kit ELISA, nlrr-6 Kit ELISA, figler3 Kit ELISA, xnlrr-1 Kit ELISA, MGC68868 Kit ELISA, XlNLRR-6 Kit ELISA, LRRN1 Kit ELISA, FIGLER3 Kit ELISA, NLRR-1 Kit ELISA, LRCH4 Kit ELISA, 2810047E21Rik Kit ELISA, leucine rich repeat neuronal 1 L homeolog Kit ELISA, leucine rich repeat neuronal 1 Kit ELISA, leucine rich repeat neuronal 1-like L homeolog Kit ELISA, leucine rich repeat neuronal 1-like Kit ELISA, leucine rich repeat protein 1, neuronal Kit ELISA, lrrn1.L Kit ELISA, lrrn1 Kit ELISA, lrrn1-like.L Kit ELISA, lrrn1-like Kit ELISA, LRRN1 Kit ELISA, Lrrn1 Kit ELISA
    ID gène
    57633
    UniProt
    Q6UXK5
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