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LTA4H Kit ELISA

LTA4H Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883676
  • Antigène Voir toutes LTA4H Kits ELISA
    LTA4H (Leukotriene A4 Hydrolase (LTA4H))
    Reactivité
    • 4
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human LTA4H ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes LTA4H Kits ELISA
    LTA4H (Leukotriene A4 Hydrolase (LTA4H))
    Autre désignation
    LTA4H (LTA4H Produits)
    Synonymes
    LTA4H Kit ELISA, zgc:85809 Kit ELISA, MGC78867 Kit ELISA, lta4h Kit ELISA, MGC69197 Kit ELISA, LOC732953 Kit ELISA, DDBDRAFT_0191291 Kit ELISA, DDBDRAFT_0216768 Kit ELISA, DDB_0191291 Kit ELISA, DDB_0216768 Kit ELISA, AmpT Kit ELISA, leukotriene A4 hydrolase Kit ELISA, leukotriene A4 hydrolase L homeolog Kit ELISA, Leukotriene A4 hydrolase Kit ELISA, LTA4H Kit ELISA, lta4h Kit ELISA, lta4h.L Kit ELISA, LOC732953 Kit ELISA, Shew185_1367 Kit ELISA, Sbal195_1406 Kit ELISA, XfasM23_0741 Kit ELISA, Sbal223_2977 Kit ELISA, lkhA Kit ELISA, Lta4h Kit ELISA
    ID gène
    4048
    UniProt
    P09960
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