Myoglobin Kit ELISA
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- Antigène Voir toutes Myoglobin (MB) Kits ELISA
- Myoglobin (MB)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.82-200 ng/mL
- Seuil minimal de détection
- 0.82 ng/mL
- Application
- ELISA
- Fonction
- Human Myoglobin ELISA Kit for serum, plasma, and cell culture supernatant samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human Myoglobin. Other species not determined.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Myoglobin (MB) Kits ELISA
- Myoglobin (MB)
- Autre désignation
- Myoglobin (MB Produits)
- Synonymes
- PVALB Kit ELISA, AI325109 Kit ELISA, zgc:65819 Kit ELISA, zgc:77764 Kit ELISA, MB Kit ELISA, DKFZp468H096 Kit ELISA, myg Kit ELISA, mb Kit ELISA, MYF4 Kit ELISA, bHLHc3 Kit ELISA, myo Kit ELISA, Myoglobin Kit ELISA, myoglobin Kit ELISA, myogenin Kit ELISA, MB Kit ELISA, Mb Kit ELISA, mb Kit ELISA, myg Kit ELISA, Myog Kit ELISA
- ID gène
- 4151
- UniProt
- P02144
- Pathways
- Brown Fat Cell Differentiation
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