PVRL1 Kit ELISA

N° du produit ABIN4883868

Détail du produit PVRL1 Kit ELISA

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Nectin-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes human Nectin-1. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Autre désignation: Nectin-1 (PVRL1 Produits)

Synonymes: CD111 Kit ELISA, CLPED1 Kit ELISA, ED4 Kit ELISA, HIgR Kit ELISA, HVEC Kit ELISA, OFC7 Kit ELISA, PRR Kit ELISA, PRR1 Kit ELISA, PVRR Kit ELISA, PVRR1 Kit ELISA, SK-12 Kit ELISA, nectin-1 Kit ELISA, prr1 Kit ELISA, PVRL1 Kit ELISA, pvrl1 Kit ELISA, AI835281 Kit ELISA, AW549174 Kit ELISA, Cd111 Kit ELISA, HveC Kit ELISA, cd111 Kit ELISA, cd112 Kit ELISA, clped1 Kit ELISA, ed4 Kit ELISA, higr Kit ELISA, hveb Kit ELISA, hvec Kit ELISA, nectin-2 Kit ELISA, ofc7 Kit ELISA, prr Kit ELISA, prr2 Kit ELISA, pvrl2 Kit ELISA, pvrl2.L Kit ELISA, pvrr Kit ELISA, pvrr1 Kit ELISA, pvrr2 Kit ELISA, sk-12 Kit ELISA, nectin cell adhesion molecule 1 Kit ELISA, nectin-1 Kit ELISA, Poliovirus receptor-related protein 1 Kit ELISA, poliovirus receptor-related 1 (herpesvirus entry mediator C) Kit ELISA, nectin cell adhesion molecule 2 L homeolog Kit ELISA, NECTIN1 Kit ELISA, LOC100145553 Kit ELISA, pvrl1 Kit ELISA, nectin1 Kit ELISA, Nectin1 Kit ELISA, PVRL1 Kit ELISA, nectin2.L Kit ELISA

ID gène: 5818

UniProt: Q15223

Pathways: Cell-Cell Junction Organization