RPS6KB1 Kit ELISA

N° du produit ABIN4884071

Détail du produit RPS6KB1 Kit ELISA

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human p70 S6 Kinase ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: p70 S6 Kinase (RPS6KB1 Produits)

Synonymes: PS6K Kit ELISA, S6K Kit ELISA, S6K-beta-1 Kit ELISA, S6K1 Kit ELISA, STK14A Kit ELISA, p70 S6KA Kit ELISA, p70(S6K)-alpha Kit ELISA, p70-S6K Kit ELISA, p70-alpha Kit ELISA, 10539 Kit ELISA, 7084 Kit ELISA, CG10539 Kit ELISA, DS6K Kit ELISA, Dmel\\CG10539 Kit ELISA, Dp70S6k Kit ELISA, Dp70[s6k] Kit ELISA, Dp70s6k Kit ELISA, S6k Kit ELISA, dS6K Kit ELISA, dS6k Kit ELISA, dp70[S6k] Kit ELISA, dp70s6k Kit ELISA, dps6k Kit ELISA, ds6k Kit ELISA, fs(3)07084 Kit ELISA, l(3)07084 Kit ELISA, p70 S6K Kit ELISA, p70/S6K Kit ELISA, p70S6K Kit ELISA, p70[S6 kinase] Kit ELISA, p70[S6K] Kit ELISA, p70[S6k] Kit ELISA, p70[S6kinase] Kit ELISA, p70s6K Kit ELISA, s6k Kit ELISA, s6k11 Kit ELISA, 2610318I15Rik Kit ELISA, 4732464A07Rik Kit ELISA, 70kDa Kit ELISA, AA959758 Kit ELISA, AI256796 Kit ELISA, AI314060 Kit ELISA, p70/85s6k Kit ELISA, p70s6k Kit ELISA, p70s6k-A Kit ELISA, p70-s6k Kit ELISA, ps6k Kit ELISA, rps6kb1 Kit ELISA, rps6kb1-A Kit ELISA, s6K1 Kit ELISA, stk14a Kit ELISA, fc51h01 Kit ELISA, wu:fc51h01 Kit ELISA, zgc:55713 Kit ELISA, ribosomal protein S6 kinase B1 Kit ELISA, Ribosomal protein S6 kinase Kit ELISA, ribosomal protein S6 kinase, polypeptide 1 Kit ELISA, ribosomal protein S6 kinase B1 L homeolog Kit ELISA, ribosomal protein S6 kinase B1 S homeolog Kit ELISA, ribosomal protein S6 kinase b, polypeptide 1b Kit ELISA, RPS6KB1 Kit ELISA, S6k Kit ELISA, Rps6kb1 Kit ELISA, rps6kb1.L Kit ELISA, rps6kb1.S Kit ELISA, rps6kb1b Kit ELISA

ID gène: 6198

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg