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PLA2G2A Kit ELISA
PLA2G2A
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884170
Détail du produit PLA2G2A Kit ELISA
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Antigène
Voir toutes PLA2G2A Kits ELISA
PLA2G2A
(Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))
Reactivité
Toutes les réactivités sur PLA2G2A Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human PLA2G2A ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human PLA2G2A.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes PLA2G2A Kits ELISA
PLA2G2A
(Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))
Autre désignation
PLA2G2A (PLA2G2A Produits )
Synonymes
MOM1 Kit ELISA, PLA2 Kit ELISA, PLA2B Kit ELISA, PLA2L Kit ELISA, PLA2S Kit ELISA, PLAS1 Kit ELISA, sPLA2 Kit ELISA, EF Kit ELISA, Mom1 Kit ELISA, Pla2 Kit ELISA, sPla2-IIA Kit ELISA, phospholipase A2 group IIA Kit ELISA, phospholipase A2, group IIA (platelets, synovial fluid) Kit ELISA, PLA2G2A Kit ELISA, Pla2g2a Kit ELISA
ID gène
5320
UniProt
P14555
Pathways
Stem Cell Maintenance , Inositol Metabolic Process
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