Plexin A4 Kit ELISA

N° du produit ABIN4884197

Détail du produit Plexin A4 Kit ELISA

Antigène: Plexin A4 (PLXNA4)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Plexin A4 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Plexin A4 (PLXNA4)

Autre désignation: Plexin A4 (PLXNA4 Produits)

Synonymes: D204 Kit ELISA, wu:fd49b01 Kit ELISA, wu:fe15f03 Kit ELISA, PLXNA4A Kit ELISA, FAYV2820 Kit ELISA, PLEXA4 Kit ELISA, PLXNA4B Kit ELISA, PRO34003 Kit ELISA, 9330117B14 Kit ELISA, Plxa4 Kit ELISA, mKIAA1550 Kit ELISA, PLEX2 Kit ELISA, Plxna4 Kit ELISA, PLXNA4 Kit ELISA, plexin A4 Kit ELISA, plexin-A4 Kit ELISA, plxna4 Kit ELISA, PLXNA4 Kit ELISA, LOC100467706 Kit ELISA, Plxna4 Kit ELISA

ID gène: 91584

UniProt: Q9HCM2

Pathways: Regulation of Cell Size