Plexin A4 Kit ELISA
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- Antigène Voir toutes Plexin A4 (PLXNA4) Kits ELISA
- Plexin A4 (PLXNA4)
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Human Plexin A4 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Plexin A4 (PLXNA4) Kits ELISA
- Plexin A4 (PLXNA4)
- Autre désignation
- Plexin A4 (PLXNA4 Produits)
- Synonymes
- D204 Kit ELISA, wu:fd49b01 Kit ELISA, wu:fe15f03 Kit ELISA, PLXNA4A Kit ELISA, FAYV2820 Kit ELISA, PLEXA4 Kit ELISA, PLXNA4B Kit ELISA, PRO34003 Kit ELISA, 9330117B14 Kit ELISA, Plxa4 Kit ELISA, mKIAA1550 Kit ELISA, PLEX2 Kit ELISA, Plxna4 Kit ELISA, PLXNA4 Kit ELISA, plexin A4 Kit ELISA, plexin-A4 Kit ELISA, plxna4 Kit ELISA, PLXNA4 Kit ELISA, LOC100467706 Kit ELISA, Plxna4 Kit ELISA
- ID gène
- 91584
- UniProt
- Q9HCM2
- Pathways
- Regulation of Cell Size
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