PON1 Kit ELISA
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- Antigène Voir toutes PON1 Kits ELISA
- PON1 (Paraoxonase 1 (PON1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human PON1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human PON1.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes PON1 Kits ELISA
- PON1 (Paraoxonase 1 (PON1))
- Autre désignation
- PON1 (PON1 Produits)
- Synonymes
- esa Kit ELISA, pon Kit ELISA, pon2 Kit ELISA, MGC53915 Kit ELISA, MGC89222 Kit ELISA, PON1 Kit ELISA, ESA Kit ELISA, MVCD5 Kit ELISA, PON Kit ELISA, Pon Kit ELISA, paraoxonase 2 Kit ELISA, paraoxonase 1 Kit ELISA, pon2 Kit ELISA, PON1 Kit ELISA, Pon1 Kit ELISA
- ID gène
- 5444
- UniProt
- P27169
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