POR Kit ELISA

N° du produit ABIN4884215

Détail du produit POR Kit ELISA

Antigène: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human POR/P450R ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: POR (P450 (Cytochrome) Oxidoreductase (POR))

Autre désignation: POR (POR Produits)

Synonymes: CPR Kit ELISA, CYPOR Kit ELISA, P450R Kit ELISA, POR Kit ELISA, CCR Kit ELISA, CG11567 Kit ELISA, DMR Kit ELISA, DmCPR Kit ELISA, Dmel\\CG11567 Kit ELISA, NCPR Kit ELISA, P450 Kit ELISA, cpr Kit ELISA, zgc:110032 Kit ELISA, npr Kit ELISA, xpor Kit ELISA, 4933424M13Rik Kit ELISA, PORCINO Kit ELISA, T5J17.90 Kit ELISA, T5J17_90 Kit ELISA, TFC C Kit ELISA, TUBULIN-FOLDING COFACTOR C Kit ELISA, cytochrome p450 oxidoreductase Kit ELISA, P450 (cytochrome) oxidoreductase Kit ELISA, ADP-ribosylation factor interacting protein 2 Kit ELISA, Cytochrome P450 reductase Kit ELISA, P450 (cytochrome) oxidoreductase a Kit ELISA, P450 (cytochrome) oxidoreductase L homeolog Kit ELISA, NADPH--cytochrome P450 reductase Kit ELISA, C-CAP/cofactor C-like domain-containing protein Kit ELISA, POR Kit ELISA, Arfip2 Kit ELISA, Por Kit ELISA, Cpr Kit ELISA, pora Kit ELISA, por.L Kit ELISA, LOC100406157 Kit ELISA, por Kit ELISA, LOC100619047 Kit ELISA

ID gène: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome