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Pyrophosphatase (Inorganic) 1 (PPA1) Kit ELISA
PPA1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884223
Détail du produit
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Antigène
Voir toutes Pyrophosphatase (Inorganic) 1 (PPA1) Kits ELISA
Pyrophosphatase (Inorganic) 1 (PPA1)
Reactivité
Toutes les réactivités sur PPA1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human PPA1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Pyrophosphatase (Inorganic) 1 (PPA1) Kits ELISA
Pyrophosphatase (Inorganic) 1 (PPA1)
Autre désignation
PPA1 (PPA1 Produits )
Synonymes
ppa Kit ELISA, SCE9.16 Kit ELISA, PSPTO0722 Kit ELISA, AO090005001437 Kit ELISA, IOPPP Kit ELISA, PP Kit ELISA, PP1 Kit ELISA, SID6-8061 Kit ELISA, 2010317E03Rik Kit ELISA, Pyp Kit ELISA, Pp Kit ELISA, inorganic pyrophosphatase Kit ELISA, Inorganic pyrophosphatase Kit ELISA, pyrophosphatase (inorganic) 1 L homeolog Kit ELISA, pyrophosphatase (inorganic) 1 Kit ELISA, ppa Kit ELISA, SCO3409 Kit ELISA, ppa-1 Kit ELISA, APE_1692.1 Kit ELISA, AOR_1_2492174 Kit ELISA, Celal_2154 Kit ELISA, Celly_1958 Kit ELISA, Dester_1338 Kit ELISA, Marky_0054 Kit ELISA, Halhy_5834 Kit ELISA, ppa1.L Kit ELISA, PPA1 Kit ELISA, Ppa1 Kit ELISA
Classe de substances
Viral Protein
ID gène
5464
UniProt
Q15181
Vus récemment
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