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SPI1 Kit ELISA
SPI1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884306
Détail du produit SPI1 Kit ELISA
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Antigène
Voir toutes SPI1 Kits ELISA
SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
Reactivité
Toutes les réactivités sur SPI1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human PU.1/Spi-1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SPI1 Kits ELISA
SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
Autre désignation
PU.1 (SPI1 Produits )
Synonymes
OF Kit ELISA, PU.1 Kit ELISA, SFPI1 Kit ELISA, SPI-1 Kit ELISA, SPI-A Kit ELISA, Dis-1 Kit ELISA, Dis1 Kit ELISA, Sfpi-1 Kit ELISA, Sfpi1 Kit ELISA, Spi-1 Kit ELISA, Tcfpu1 Kit ELISA, Tfpu.1 Kit ELISA, Pu.1 Kit ELISA, cb1086 Kit ELISA, pu.1 Kit ELISA, pu1 Kit ELISA, sfpi1 Kit ELISA, si:by184l24.2 Kit ELISA, spi1 Kit ELISA, SPI1/PU.1 Kit ELISA, spi1l Kit ELISA, zgc:152991 Kit ELISA, Spi-1 proto-oncogene Kit ELISA, spleen focus forming virus (SFFV) proviral integration oncogene Kit ELISA, Spi-1 proto-oncogene b Kit ELISA, Spi-1 proto-oncogene a Kit ELISA, SPI1 Kit ELISA, Spi1 Kit ELISA, spi1b Kit ELISA, spi1a Kit ELISA
ID gène
6688
Pathways
Stem Cell Maintenance
Vus récemment
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