ROBO2 Kit ELISA

N° du produit ABIN4884365

Détail du produit ROBO2 Kit ELISA

Antigène: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human ROBO2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human ROBO2. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Autre désignation: ROBO2 (ROBO2 Produits)

Synonymes: SAX3 Kit ELISA, ROBO2 Kit ELISA, CG14347 Kit ELISA, CG14348 Kit ELISA, CG5481 Kit ELISA, CG5574 Kit ELISA, CT17326 Kit ELISA, D-Robo2 Kit ELISA, D-robo2 Kit ELISA, Dmel\\CG5481 Kit ELISA, Robo 2 Kit ELISA, Robo-2 Kit ELISA, Robo2 Kit ELISA, anon-EST:Liang-1.75 Kit ELISA, clone 1.75 Kit ELISA, dRobo-2 Kit ELISA, fus4 Kit ELISA, robo-2 Kit ELISA, robo2 Kit ELISA, unp1881 Kit ELISA, 2600013A04Rik Kit ELISA, 9430089E08Rik Kit ELISA, BB097918 Kit ELISA, D230004I22Rik Kit ELISA, mKIAA1568 Kit ELISA, sax3 Kit ELISA, roundabout guidance receptor 2 Kit ELISA, roundabout 2 Kit ELISA, roundabout, axon guidance receptor, homolog 2 (Drosophila) Kit ELISA, roundabout guidance receptor 2 S homeolog Kit ELISA, ROBO2 Kit ELISA, Robo2 Kit ELISA, robo2 Kit ELISA, robo2.S Kit ELISA

ID gène: 6092

UniProt: Q9HCK4

Pathways: Regulation of Cell Size