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SPRY1 Kit ELISA
SPRY1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884641
Détail du produit SPRY1 Kit ELISA
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Antigène
Tous les produits SPRY1
SPRY1
(Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human SPRY1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Tous les produits SPRY1
SPRY1
(Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
Autre désignation
SPRY1 (SPRY1 Produits )
Synonymes
hSPRY1 Kit ELISA, SPRY1 Kit ELISA, LOC100223875 Kit ELISA, hspry1 Kit ELISA, xspry1 Kit ELISA, sprouty Kit ELISA, sprouty1 Kit ELISA, sprouty-1 Kit ELISA, Xtsprouty1 Kit ELISA, spry1a Kit ELISA, spry1b Kit ELISA, sprouty RTK signaling antagonist 1 Kit ELISA, sprouty RTK signaling antagonist 1 L homeolog Kit ELISA, sprouty RTK signaling antagonist 1 S homeolog Kit ELISA, sprouty homolog 1 (Drosophila) Kit ELISA, SPRY1 Kit ELISA, Spry1 Kit ELISA, spry1 Kit ELISA, spry1.L Kit ELISA, spry1.S Kit ELISA
ID gène
10252
UniProt
O43609
Pathways
EGFR Signaling Pathway , EGFR Downregulation
Vus récemment
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