Src Kit ELISA

N° du produit ABIN4884647

Détail du produit Src Kit ELISA

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Src ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Autre désignation: Src (Src Produits)

Synonymes: AW259666 Kit ELISA, pp60c-src Kit ELISA, C-src1 Kit ELISA, CG7524 Kit ELISA, D-Src64B Kit ELISA, D-src Kit ELISA, DSRC64 Kit ELISA, DSrc Kit ELISA, DSrc64 Kit ELISA, DSrc64B Kit ELISA, Dm SRC1 Kit ELISA, DmHD-358 Kit ELISA, Dmel\CG7524 Kit ELISA, Dsrc Kit ELISA, Dsrc64 Kit ELISA, Dsrc64B Kit ELISA, HD-358 Kit ELISA, MRE5 Kit ELISA, SRC 64B Kit ELISA, Src Kit ELISA, Src1 Kit ELISA, Src64 Kit ELISA, c-src Kit ELISA, dSrc Kit ELISA, dsrc Kit ELISA, src Kit ELISA, src1 Kit ELISA, src64 Kit ELISA, src64B Kit ELISA, src64b Kit ELISA, ASV Kit ELISA, SRC1 Kit ELISA, c-SRC Kit ELISA, p60-Src Kit ELISA, c-Src Kit ELISA, PP60C-SCR Kit ELISA, SDR Kit ELISA, SRC proto-oncogene, non-receptor tyrosine kinase Kit ELISA, uncharacterized LOC5574802 Kit ELISA, proto-oncogene tyrosine-protein kinase src Kit ELISA, Rous sarcoma oncogene Kit ELISA, Src oncogene at 64B Kit ELISA, SRC Kit ELISA, LOC5574802 Kit ELISA, CpipJ_CPIJ010659 Kit ELISA, Src Kit ELISA, Src64B Kit ELISA

Classe de substances: Viral Protein

ID gène: 6714

UniProt: P12931

Pathways: Signalistation JAK/STAT, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling