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SULT1B1 Kit ELISA

SULT1B1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884686
  • Antigène Voir toutes SULT1B1 Kits ELISA
    SULT1B1 (Sulfotransferase Family 1B Member 1 (SULT1B1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human SULT1B1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes SULT1B1 Kits ELISA
    SULT1B1 (Sulfotransferase Family 1B Member 1 (SULT1B1))
    Autre désignation
    SULT1B1 (SULT1B1 Produits)
    Synonymes
    ST1B1 Kit ELISA, ST1B2 Kit ELISA, SULT1B2 Kit ELISA, St2b2 Kit ELISA, SULT1B Kit ELISA, cSULT1B1 Kit ELISA, MGC80677 Kit ELISA, SULT1B1 Kit ELISA, sulfotransferase family 1B member 1 Kit ELISA, sulfotransferase family 1B, member 1 Kit ELISA, sulfotransferase family cytosolic 1B member 1 Kit ELISA, sulfotransferase family 1B member 1 S homeolog Kit ELISA, sulfotransferase family, cytosolic, 1B, member 1 Kit ELISA, SULT1B1 Kit ELISA, Sult1b1 Kit ELISA, sult1b1.S Kit ELISA, sult1b1 Kit ELISA, LOC100624541 Kit ELISA
    ID gène
    27284
    UniProt
    O43704
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