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Transferrin Receptor Kit ELISA
TFRC
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.819-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884758
Détail du produit Transferrin Receptor Kit ELISA
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Antigène
Voir toutes Transferrin Receptor (TFRC) Kits ELISA
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Reactivité
Toutes les réactivités sur Transferrin Receptor Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.819-200 ng/mL
Seuil minimal de détection
0.819 ng/mL
Application
ELISA
Fonction
Human TfR/Transferrin R/CD71 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human TfR.
Sensibilité
0.81 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples200 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Transferrin Receptor (TFRC) Kits ELISA
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Autre désignation
TfR (Transferrin R/CD71) (TFRC Produits )
Synonymes
Trfr Kit ELISA, LOC100219280 Kit ELISA, tfr Kit ELISA, cd71 Kit ELISA, tfr1 Kit ELISA, trfr Kit ELISA, CD71 Kit ELISA, T9 Kit ELISA, TFR Kit ELISA, TFR1 Kit ELISA, TR Kit ELISA, TRFR Kit ELISA, p90 Kit ELISA, 2610028K12Rik Kit ELISA, AI195355 Kit ELISA, AI426448 Kit ELISA, AU015758 Kit ELISA, E430033M20Rik Kit ELISA, Mtvr-1 Kit ELISA, Mtvr1 Kit ELISA, transferrin receptor Kit ELISA, TFRC Kit ELISA, Tfrc Kit ELISA, tfrc Kit ELISA
ID gène
7037
UniProt
P02786
Pathways
Transition Metal Ion Homeostasis
Vus récemment
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