Thimet Oligopeptidase 1 Kit ELISA

N° du produit ABIN4884783

Détail du produit Thimet Oligopeptidase 1 Kit ELISA

Antigène: Thimet Oligopeptidase 1 (THOP1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Thimet Oligopeptidase (THOP1) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Thimet Oligopeptidase 1 (THOP1)

Autre désignation: Thimet Oligopeptidase (THOP1 Produits)

Synonymes: EP24.15 Kit ELISA, MEPD_HUMAN Kit ELISA, MP78 Kit ELISA, TOP Kit ELISA, AI131655 Kit ELISA, AI327041 Kit ELISA, THOP1 Kit ELISA, thop1 Kit ELISA, MGC147297 Kit ELISA, LOC397806 Kit ELISA, mepd Kit ELISA, thimet oligopeptidase 1 Kit ELISA, thimet oligopeptidase 1 L homeolog Kit ELISA, thimet oligopeptidase Kit ELISA, peptidase Kit ELISA, Thimet oligopeptidase Kit ELISA, THOP1 Kit ELISA, Thop1 Kit ELISA, thop1 Kit ELISA, thop1.L Kit ELISA, Tc00.1047053506411.10 Kit ELISA, Tc00.1047053511237.10 Kit ELISA, Noca_4613 Kit ELISA, ANI_1_1400064 Kit ELISA, MBOO_RS10705 Kit ELISA, AOR_1_880174 Kit ELISA, AnaeK_0425 Kit ELISA, MPAL_RS03920 Kit ELISA, Hoch_5061 Kit ELISA, mepd Kit ELISA

ID gène: 7064

UniProt: P52888