Thymopoietin Kit ELISA

N° du produit ABIN4884832

Détail du produit Thymopoietin Kit ELISA

Antigène: Thymopoietin (TMPO)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Thymopoietin (LAP2) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Thymopoietin (TMPO)

Autre désignation: Thymopoietin (TMPO Produits)

Synonymes: LAP2 Kit ELISA, CMD1T Kit ELISA, LEMD4 Kit ELISA, PRO0868 Kit ELISA, TP Kit ELISA, lap2 Kit ELISA, LAP2beta Kit ELISA, 5630400D24Rik Kit ELISA, AI195756 Kit ELISA, AI606875 Kit ELISA, AW214352 Kit ELISA, AW547477 Kit ELISA, tmpo Kit ELISA, wu:fc26f12 Kit ELISA, wu:fd21f02 Kit ELISA, wu:fi29h05 Kit ELISA, thymopoietin Kit ELISA, thymopoietin S homeolog Kit ELISA, thymopoietin a Kit ELISA, TMPO Kit ELISA, Tmpo Kit ELISA, tmpo.S Kit ELISA, tmpoa Kit ELISA

ID gène: 7112