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TRKA Kit ELISA
NTRK1
Reactivité: Humain
Colorimetric
Sandwich ELISA
2-500 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884901
Détail du produit TRKA Kit ELISA
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Antigène
Voir toutes TRKA (NTRK1) Kits ELISA
TRKA (NTRK1)
(Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
Reactivité
Toutes les réactivités sur TRKA Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
2-500 ng/mL
Seuil minimal de détection
2 ng/mL
Application
ELISA
Fonction
Human TrkA ELISA Kit for serum, plasma, and cell culture supernatant samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects human TrkA. Other species not determined.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TRKA (NTRK1) Kits ELISA
TRKA (NTRK1)
(Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
Autre désignation
TrkA (NTRK1 Produits )
Synonymes
MTC Kit ELISA, TRK Kit ELISA, TRK1 Kit ELISA, TRKA Kit ELISA, Trk-A Kit ELISA, p140-TrkA Kit ELISA, CDHF12 Kit ELISA, CDHR16 Kit ELISA, HSCR1 Kit ELISA, MEN2A Kit ELISA, MEN2B Kit ELISA, MTC1 Kit ELISA, PTC Kit ELISA, RET-ELE1 Kit ELISA, RET51 Kit ELISA, Trk Kit ELISA, C80751 Kit ELISA, Tkr Kit ELISA, TrkA Kit ELISA, trk Kit ELISA, trka Kit ELISA, CTRKA Kit ELISA, trkA Kit ELISA, neurotrophic receptor tyrosine kinase 1 Kit ELISA, ret proto-oncogene Kit ELISA, neurotrophic tyrosine kinase, receptor, type 1 Kit ELISA, NTRK1 Kit ELISA, RET Kit ELISA, Ntrk1 Kit ELISA, ntrk1 Kit ELISA
ID gène
4914
UniProt
P04629
Pathways
Signalisation RTK , Neurotrophin Signaling Pathway , cAMP Metabolic Process
Vus récemment
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