UGT1A1 Kit ELISA

N° du produit ABIN4884976

Détail du produit UGT1A1 Kit ELISA

Antigène: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human UGT1A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Autre désignation: UGT1A1 (UGT1A1 Produits)

Synonymes: BILIQTL1 Kit ELISA, GNT1 Kit ELISA, HUG-BR1 Kit ELISA, UDPGT Kit ELISA, UDPGT 1-1 Kit ELISA, UGT1 Kit ELISA, UGT1A Kit ELISA, Gnt1 Kit ELISA, UGT1A01 Kit ELISA, Udpgt-1a Kit ELISA, UgtBr1 Kit ELISA, Udpgt Kit ELISA, Ugt1 Kit ELISA, zgc:123097 Kit ELISA, UDP glucuronosyltransferase family 1 member A1 Kit ELISA, UDP glucuronosyltransferase 1 family, polypeptide A1 Kit ELISA, UDP-glucuronosyltransferase 1-1 Kit ELISA, UDP-glucuronosyltransferase Kit ELISA, UDP-glucuronosyltransferase 1-6 Kit ELISA, UDP glucuronosyltransferase 1 family polypeptide a1 Kit ELISA, UGT1A1 Kit ELISA, Ugt1a1 Kit ELISA, LOC100065342 Kit ELISA, LOC100125517 Kit ELISA, LOC100229734 Kit ELISA, LOC100405984 Kit ELISA, LOC100511479 Kit ELISA, ugt1a1 Kit ELISA

ID gène: 54658

UniProt: P22309

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha