VAPB Kit ELISA

N° du produit ABIN4885020

Détail du produit VAPB Kit ELISA

Antigène: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human VAP-B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Autre désignation: VAP-B (VAPB Produits)

Synonymes: ALS8 Kit ELISA, VAMP-B Kit ELISA, VAP-B Kit ELISA, AI225786 Kit ELISA, AI840687 Kit ELISA, AI848259 Kit ELISA, D2Abb2e Kit ELISA, R75548 Kit ELISA, VAP33b Kit ELISA, Vamp33b Kit ELISA, fc05d09 Kit ELISA, wu:fa96d05 Kit ELISA, wu:fc05d09 Kit ELISA, zgc:66045 Kit ELISA, vamp-b Kit ELISA, vamp-c Kit ELISA, vap-b Kit ELISA, vap-c Kit ELISA, vapb Kit ELISA, VAMP associated protein B and C Kit ELISA, vesicle-associated membrane protein-associated protein B/C Kit ELISA, vesicle-associated membrane protein, associated protein B and C Kit ELISA, VAMP (vesicle-associated membrane protein)-associated protein B and C Kit ELISA, VAMP (vesicle-associated membrane protein)-associated protein B and C L homeolog Kit ELISA, VAPB Kit ELISA, Tsp_07972 Kit ELISA, Vapb Kit ELISA, vapb Kit ELISA, vapb.L Kit ELISA

ID gène: 9217

UniProt: O95292

Pathways: ER-Nucleus Signaling