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Transferrin Receptor Kit ELISA
TFRC
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885348
Détail du produit Transferrin Receptor Kit ELISA
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Antigène
Voir toutes Transferrin Receptor (TFRC) Kits ELISA
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Reactivité
Toutes les réactivités sur Transferrin Receptor Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human TfR (Transferrin R) SpeedELISA Kit.
Marque
SpeedELISA
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Attributs du produit
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Durée du test
3 h
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Transferrin Receptor (TFRC) Kits ELISA
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Autre désignation
TfR (TFRC Produits )
Synonymes
Trfr Kit ELISA, LOC100219280 Kit ELISA, tfr Kit ELISA, cd71 Kit ELISA, tfr1 Kit ELISA, trfr Kit ELISA, CD71 Kit ELISA, T9 Kit ELISA, TFR Kit ELISA, TFR1 Kit ELISA, TR Kit ELISA, TRFR Kit ELISA, p90 Kit ELISA, 2610028K12Rik Kit ELISA, AI195355 Kit ELISA, AI426448 Kit ELISA, AU015758 Kit ELISA, E430033M20Rik Kit ELISA, Mtvr-1 Kit ELISA, Mtvr1 Kit ELISA, transferrin receptor Kit ELISA, TFRC Kit ELISA, Tfrc Kit ELISA, tfrc Kit ELISA
ID gène
7037
UniProt
P02786
Pathways
Transition Metal Ion Homeostasis
Vus récemment
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