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CCL28 Kit ELISA

CCL28 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885454
  • Antigène Voir toutes CCL28 Kits ELISA
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Reactivité
    • 5
    • 4
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Mouse Chemokine (C-C motif) Ligand 28 (MEC) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Mouse Chemokine (C-C motif) Ligand 28 (MEC)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CCL28 Kits ELISA
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Autre désignation
    CCL28 (CCL28 Produits)
    Synonymes
    Scya28 Kit ELISA, CCL28 Kit ELISA, ccl28 Kit ELISA, nrpb Kit ELISA, enc-1 Kit ELISA, pig10 Kit ELISA, Encr-3 Kit ELISA, klhl35 Kit ELISA, Xencr-3 Kit ELISA, tp53i10 Kit ELISA, LOC100303767 Kit ELISA, CCK1 Kit ELISA, MEC Kit ELISA, SCYA28 Kit ELISA, C-C motif chemokine ligand 28 Kit ELISA, ectodermal-neural cortex 1 (with BTB domain) Kit ELISA, C-C motif chemokine 28 Kit ELISA, chemokine (C-C motif) ligand 28 Kit ELISA, Ccl28 Kit ELISA, CCL28 Kit ELISA, ccl28 Kit ELISA, enc1 Kit ELISA, LOC100303767 Kit ELISA
    ID gène
    56838
    UniProt
    Q9JIL2
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