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KREMEN1 Kit ELISA
KREMEN1
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885592
Détail du produit KREMEN1 Kit ELISA
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Antigène
Voir toutes KREMEN1 Kits ELISA
KREMEN1
(Kringle Containing Transmembrane Protein 1 (KREMEN1))
Reactivité
Toutes les réactivités sur KREMEN1 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Mouse Kremen-1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes mouse Kremen-1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes KREMEN1 Kits ELISA
KREMEN1
(Kringle Containing Transmembrane Protein 1 (KREMEN1))
Autre désignation
Kremen-1 (KREMEN1 Produits )
Synonymes
KREMEN1 Kit ELISA, krm1 Kit ELISA, kremen Kit ELISA, kremem1 Kit ELISA, KREMEN Kit ELISA, KRM1 Kit ELISA, AV002070 Kit ELISA, Kremen Kit ELISA, Krm1 Kit ELISA, si:ct737139.1 Kit ELISA, si:dkeyp-7c9.1 Kit ELISA, kringle containing transmembrane protein 1 Kit ELISA, kringle containing transmembrane protein 1 S homeolog Kit ELISA, KREMEN1 Kit ELISA, kremen1 Kit ELISA, Kremen1 Kit ELISA, kremen1.S Kit ELISA
ID gène
84035
UniProt
Q99N43
Pathways
Signalisation WNT
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