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SPOCK3 Kit ELISA
SPOCK3
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885655
Détail du produit SPOCK3 Kit ELISA
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Antigène
Voir toutes SPOCK3 Kits ELISA
SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
Reactivité
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Mouse Testican 3 (SPOCK) ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes mouse Testican 3/SPOCK.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SPOCK3 Kits ELISA
SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
Autre désignation
Testican 3 (SPOCK3 Produits )
Synonymes
im:7139568 Kit ELISA, si:dkey-285b22.1 Kit ELISA, HSAJ1454 Kit ELISA, TES-3 Kit ELISA, TICN3 Kit ELISA, Testican-3 Kit ELISA, 2900045C01Rik Kit ELISA, AI428471 Kit ELISA, mKIAA4039 Kit ELISA, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 Kit ELISA, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 Kit ELISA, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 Kit ELISA, spock3 Kit ELISA, SPOCK3 Kit ELISA, Spock3 Kit ELISA
ID gène
72902
UniProt
Q8BKV0
Pathways
Glycosaminoglycan Metabolic Process
Vus récemment
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