AXL Kit ELISA
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- Antigène Voir toutes AXL Kits ELISA
- AXL (AXL Receptor tyrosine Kinase (AXL))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Mouse AXL SpeedELISA Kit.
- Marque
- SpeedELISA
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Attributs du produit
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - Date de péremption
- 6 months
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- Antigène Voir toutes AXL Kits ELISA
- AXL (AXL Receptor tyrosine Kinase (AXL))
- Autre désignation
- AXL (AXL Produits)
- Synonymes
- JTK11 Kit ELISA, UFO Kit ELISA, AI323647 Kit ELISA, Ark Kit ELISA, Tyro7 Kit ELISA, Ufo Kit ELISA, AXL receptor tyrosine kinase Kit ELISA, Axl receptor tyrosine kinase Kit ELISA, AXL Kit ELISA, Axl Kit ELISA
- ID gène
- 26362
- UniProt
- Q00993
- Pathways
- Signalisation RTK, Cellular Response to Molecule of Bacterial Origin
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