FABP1 Kit ELISA

N° du produit ABIN4885866

Détail du produit FABP1 Kit ELISA

Antigène: FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat FABP-1..

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

Autre désignation: FABP1 (FABP1 Produits)

Synonymes: FABPL Kit ELISA, L-FABP Kit ELISA, KAT4 Kit ELISA, KATIV Kit ELISA, mitAAT Kit ELISA, Fabpl Kit ELISA, Fabplg Kit ELISA, SCP Kit ELISA, SCP. Kit ELISA, p14 Kit ELISA, FABP1 Kit ELISA, FABP Kit ELISA, LFABP Kit ELISA, liver Kit ELISA, fabp1 Kit ELISA, Fabp1 Kit ELISA, FABP-1 Kit ELISA, FABPpm Kit ELISA, mAspAT Kit ELISA, fatty acid binding protein 1 Kit ELISA, glutamic-oxaloacetic transaminase 2 Kit ELISA, fatty acid binding protein 1, liver Kit ELISA, fatty acid binding protein 1a, liver Kit ELISA, fatty acid-binding protein, liver Kit ELISA, FABP1 Kit ELISA, GOT2 Kit ELISA, Fabp1 Kit ELISA, fabp1a Kit ELISA, LOC100726384 Kit ELISA

ID gène: 25721

UniProt: P00507

Pathways: Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha