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DIABLO Kit ELISA
DIABLO
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885896
Détail du produit DIABLO Kit ELISA
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Antigène
Voir toutes DIABLO Kits ELISA
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Reactivité
Toutes les réactivités sur DIABLO Kits ELISA
Rat
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Rat SMAC ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Rat SMAC.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes DIABLO Kits ELISA
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Autre désignation
SMAC (DIABLO Produits )
Synonymes
smac Kit ELISA, smac3 Kit ELISA, diablo-s Kit ELISA, 0610041G12Rik Kit ELISA, 1700006L01Rik Kit ELISA, AU040403 Kit ELISA, Smac Kit ELISA, DFNA64 Kit ELISA, SMAC Kit ELISA, DBO Kit ELISA, XSmac Kit ELISA, diablo, IAP-binding mitochondrial protein Kit ELISA, diablo IAP-binding mitochondrial protein Kit ELISA, diablo, IAP-binding mitochondrial protein L homeolog Kit ELISA, diablo Kit ELISA, Diablo Kit ELISA, DIABLO Kit ELISA, diablo.L Kit ELISA
ID gène
288753
UniProt
Q5RK17
Pathways
Apoptose , Caspase Cascade in Apoptosis , Positive Regulation of Endopeptidase Activity
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