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Acetyl-CoA Carboxylase alpha Kit ELISA

ACACA Reactivité: Humain, Rat pSer79 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4885960
  • Antigène Voir toutes Acetyl-CoA Carboxylase alpha (ACACA) Kits ELISA
    Acetyl-CoA Carboxylase alpha (ACACA)
    Épitope
    pSer79, total
    Reactivité
    • 7
    • 7
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Rat Phospho-ACC1 (S79) and Total ACC1 ELISA Kit. This assay semi-quantitatively measures phophorylated ACC1 (Ser79) and Total ACC1 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human, and rat ACC1 phosphorylated at site Serine-79 and Total ACC1.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes Acetyl-CoA Carboxylase alpha (ACACA) Kits ELISA
    Acetyl-CoA Carboxylase alpha (ACACA)
    Autre désignation
    ACC1 (ACACA Produits)
    Synonymes
    ACAC Kit ELISA, ACACAD Kit ELISA, ACC Kit ELISA, ACC1 Kit ELISA, ACCA Kit ELISA, A530025K05Rik Kit ELISA, Acac Kit ELISA, Acc1 Kit ELISA, Gm738 Kit ELISA, AT-ACC1 Kit ELISA, EMB22 Kit ELISA, EMBRYO DEFECTIVE 22 Kit ELISA, GK Kit ELISA, GURKE Kit ELISA, PAS3 Kit ELISA, PASTICCINO 3 Kit ELISA, acetyl-CoA carboxylase 1 Kit ELISA, acc Kit ELISA, fj43d01 Kit ELISA, im:7138837 Kit ELISA, si:ch211-199d18.1 Kit ELISA, wu:fj43d01 Kit ELISA, acetyl-CoA carboxylase alpha Kit ELISA, acetyl-Coenzyme A carboxylase alpha Kit ELISA, acetyl-CoA carboxylase 1 Kit ELISA, ACACA Kit ELISA, Acaca Kit ELISA, ACC1 Kit ELISA, acaca Kit ELISA
    ID gène
    31
    UniProt
    Q13085
    Pathways
    AMPK Signaling, Ribonucleoside Biosynthetic Process, L'effet Warburg
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