ATM Kit ELISA

N° du produit ABIN4885984

Détail du produit ATM Kit ELISA

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Épitope: pSer1981, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-ATM (S1981) and Total ATM ELISA Kit. This assay semi-quantitatively measures phophorylated ATM (Ser1981) and Total ATM in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human ATM phosphorylated at site Serine-1981 as well as total ATM.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: ATM (ATM Produits)

Synonymes: ATM Kit ELISA, Atm Kit ELISA, CG6535 Kit ELISA, Dmel\\CG6535 Kit ELISA, Tefu Kit ELISA, atm Kit ELISA, atm/tefu Kit ELISA, dATM Kit ELISA, tef Kit ELISA, Xatm Kit ELISA, at1 Kit ELISA, atdc Kit ELISA, tel1 Kit ELISA, telo1 Kit ELISA, AT1 Kit ELISA, ATA Kit ELISA, ATC Kit ELISA, ATD Kit ELISA, ATDC Kit ELISA, ATE Kit ELISA, TEL1 Kit ELISA, TELO1 Kit ELISA, AI256621 Kit ELISA, C030026E19Rik Kit ELISA, telomere fusion Kit ELISA, ATM serine/threonine kinase L homeolog Kit ELISA, ATM serine/threonine kinase Kit ELISA, ataxia telangiectasia mutated Kit ELISA, ataxia telangiectasia mutated (atm) Kit ELISA, serine/threonine-protein kinase ATM Kit ELISA, tefu Kit ELISA, atm.L Kit ELISA, atm Kit ELISA, ATM Kit ELISA, EDI_100660 Kit ELISA, CpipJ_CPIJ001772 Kit ELISA, BDBG_08252 Kit ELISA, PAAG_02532 Kit ELISA, MCYG_05088 Kit ELISA, VDBG_06833 Kit ELISA, ACLA_015700 Kit ELISA, LOC5565620 Kit ELISA, MGYG_07634 Kit ELISA, PGTG_14279 Kit ELISA, Atm Kit ELISA

ID gène: 472

UniProt: Q13315

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus