CSK Kit ELISA
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- Antigène Voir toutes CSK Kits ELISA
- CSK (C-Src tyrosine Kinase (CSK))
- Épitope
- pTyr
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Human Phosphotyrosine CSK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine CSK in lysate samples.
- Type d'échantillon
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-CSK.
- Attributs du produit
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
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- Antigène Voir toutes CSK Kits ELISA
- CSK (C-Src tyrosine Kinase (CSK))
- Autre désignation
- CSK (CSK Produits)
- Synonymes
- AW212630 Kit ELISA, C-terminal Src kinase Kit ELISA, c-src tyrosine kinase Kit ELISA, CSK, non-receptor tyrosine kinase Kit ELISA, CSK Kit ELISA, Csk Kit ELISA
- ID gène
- 2444
- UniProt
- P41240
- Pathways
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
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