EIF4E Kit ELISA

N° du produit ABIN4886050

Détail du produit EIF4E Kit ELISA

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Épitope: pSer209, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-eIF-4E (S209) and Total eIF-4E ELISA Kit. This assay semi-quantitatively measures phophorylated eIF-4E (Ser209) and Total eIF-4E in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human eIF-4E phosphorylated at site Serine-209 and total eIF-4E.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: eIF-4E (EIF4E Produits)

Synonymes: AUTS19 Kit ELISA, CBP Kit ELISA, EIF4E1 Kit ELISA, EIF4EL1 Kit ELISA, EIF4F Kit ELISA, eif4e-1 Kit ELISA, zgc:86680 Kit ELISA, EG668879 Kit ELISA, Eif4e-ps Kit ELISA, If4e Kit ELISA, eIF-4E Kit ELISA, 0260/09 Kit ELISA, 0587/11 Kit ELISA, 0589/11 Kit ELISA, 0919/12 Kit ELISA, 1004/13 Kit ELISA, 2 Kit ELISA, 7238 Kit ELISA, CG4035 Kit ELISA, CT13384 Kit ELISA, CT39424 Kit ELISA, CT39426 Kit ELISA, D-eIF4E Kit ELISA, Dmel\\CG4035 Kit ELISA, Eif4E Kit ELISA, Eif4e Kit ELISA, IF4E Kit ELISA, dEif4e Kit ELISA, deIF-4E Kit ELISA, deIF4E Kit ELISA, eIF 4E Kit ELISA, eIF-4E1 Kit ELISA, eIF-4E2 Kit ELISA, eIF-4EII Kit ELISA, eIF-4e Kit ELISA, eIF4E Kit ELISA, eIF4E-1 Kit ELISA, eIF4E-1/2 Kit ELISA, eIF4E-2 Kit ELISA, eIF4E1 Kit ELISA, eIF4EI Kit ELISA, eIF4e Kit ELISA, eif-4E Kit ELISA, eif-4e Kit ELISA, eif4e Kit ELISA, l(3)07238 Kit ELISA, l(3)67Af Kit ELISA, l(3)S025007 Kit ELISA, l(3)S026009 Kit ELISA, l(3)S058711 Kit ELISA, l(3)S091912 Kit ELISA, cbp Kit ELISA, eif4f Kit ELISA, eif4e1 Kit ELISA, eif4el1 Kit ELISA, EIF-4E Kit ELISA, zgc:101581 Kit ELISA, eukaryotic translation initiation factor 4E Kit ELISA, eukaryotic translation initiation factor 4ea Kit ELISA, Eukaryotic initiation factor 4E Kit ELISA, eucaryotic initiation factor 6 Kit ELISA, eukaryotic translation initiation factor 4E L homeolog Kit ELISA, eukaryotic translation initiation factor 4E family member 1B S homeolog Kit ELISA, translation initiation factor eIF4E Kit ELISA, eukaryotic translation initiation factor 4eb Kit ELISA, EIF4E Kit ELISA, eif4ea Kit ELISA, Eif4e Kit ELISA, eIF-4E Kit ELISA, eif6 Kit ELISA, eif4e Kit ELISA, eif4e.L Kit ELISA, eif4e1b.S Kit ELISA, LOC100533326 Kit ELISA, eif4eb Kit ELISA

ID gène: 3287

UniProt: P06730

Pathways: BCR Signaling