EPH Receptor B1 Kit ELISA

N° du produit ABIN4886074

Détail du produit EPH Receptor B1 Kit ELISA

Antigène: EPH Receptor B1 (EPHB1)

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Phosphotyrosine EphB1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphB1 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphB1.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor B1 (EPHB1)

Autre désignation: EphB1 (EPHB1 Produits)

Synonymes: ELK Kit ELISA, EPHT2 Kit ELISA, Hek6 Kit ELISA, NET Kit ELISA, ephb1-a Kit ELISA, xek Kit ELISA, Xek Kit ELISA, MGC89790 Kit ELISA, EPHB1 Kit ELISA, CEK6 Kit ELISA, EK6 Kit ELISA, 9330129L11 Kit ELISA, AW488255 Kit ELISA, C130099E04Rik Kit ELISA, Cek6 Kit ELISA, ENSMUSG00000074119 Kit ELISA, Elk Kit ELISA, Elkh Kit ELISA, Net Kit ELISA, Ephb2 Kit ELISA, Erk Kit ELISA, elk Kit ELISA, EPH receptor B1 Kit ELISA, EPH receptor B1 S homeolog Kit ELISA, ephrin type-B receptor 1 Kit ELISA, Eph receptor B1 Kit ELISA, EPHB1 Kit ELISA, ephb1.S Kit ELISA, ephb1 Kit ELISA, LOC100467687 Kit ELISA, Ephb1 Kit ELISA

ID gène: 2047

UniProt: P54762

Pathways: Signalisation RTK